several key influences are explored. The AtMT t2 coding sequence was amplified from an Arabidopsis thaliana leaf cDNA library and cloned into the pGEX3X plasmid to allow expression of the protein as a fusion to GST in Escherichia coli. Zinc was associated with the AtMT t2 moiety of the fusion protein. The pH of 50 % displacement for the GST AtMT t2 fusion protein with respect to zinc was 0.25 pH units lower than that for the GST PsMT(_A) fusion protein indicating that putative metallothionein like protein from Arabidopsis thaliana may have a higher affinity for zinc.
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